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11-02-2020
Biology

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A binding protein binds to a ligand, L, with a Kd of 400 nM. How much ligand (in nM) is present when the fraction of ligand bound is 60%?

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olumidechemeng olumidechemeng

13-02-2020

Answer:

600nM

Explanation:

From Concentration of Ligand = Ykd/1 - Y

where Kd = 400nM.

fraction of ligand bound Y = 0.60

Plugging the values of Y and Kd;

Concentration = 0.6 x 400 / 1 - 0.6

Concentration = 600 x 10^-9

= 600nM

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